A vesicle organization process that takes place as part of the Cvt pathway, and results in the formation of a double membrane-bounded cytosolic structure that sequesters precursor aminopeptidase I (prAPI). [ http://www.ncbi.nlm.nih.gov/pubmed/11085977 GOC:rb http://www.ncbi.nlm.nih.gov/pubmed/10966461 ]
Synonyms: cytoplasm-to-vacuole targetin vesicle assembly Cvt vesicle formation cytoplasm to vacuole targeting vesicle assembly