Removal of PI3P and Atg8/LC3 after the closure of the phagophore and before the fusion with the endosome/lysosome (e.g. mammals and insects) or vacuole (yeast), and that very likely destabilizes other Atg proteins and thus enables their efficient dissociation and recycling. [ http://www.ncbi.nlm.nih.gov/pubmed/28077293 GOC:autophagy GOC:lf ]