Enzyme systems containing three different subunits and requiring ATP, S-adenosylmethionine, and magnesium for endonucleolytic activity to give random double-stranded fragments with terminal 5'-phosphates. They function also as DNA-dependent ATPases and modification methylases, catalyzing the reactions of EC 2.1.1.72 and EC 2.1.1.73 with similar site-specificity. The systems recognize specific short DNA sequences and cleave at sites remote from the recognition sequence. Enzymes from different microorganisms with the same specificity are called isoschizomers. EC 3.1.21.3.

Synonyms: Restriction Endonucleases, Type I, Type I Site-Specific DNase, DNase, Site Specific, Type I, Type I Site-Specific Deoxyribonucleases, Deoxyribonucleases, Type I Site Specific, Site-Specific DNase, Type I, Deoxyribonucleases, Type I, Site-Specific, DNA Restriction Enzymes, Type I, Deoxyribonucleases, Type I Site-Specific, Type I Site Specific DNase, Site Specific DNase, Type I, Type I Restriction Enzymes, Type I Site Specific Deoxyribonucleases, Deoxyribonucleases, Type I, Site Specific, EC 3.1.21.3, DNase, Site-Specific, Type I